Journal: Neuroscience

Article Title: Extracellular Protons Mediate Presynaptic Homeostatic Potentiation at the Mouse Neuromuscular Junction

doi: 10.1016/j.neuroscience.2021.01.036

Figure Lengend Snippet: ASIC1 and ASIC2a are located at the mouse NMJ. A. All of the images shown are maximum projections of 16 images collected at 0.5 μm increments vertically through the field containing the NMJ. α-bungarotoxin (α -BTX) is shown in Red. ASIC2a antibodies are in Green. Arrows point to clusters of ASIC2a staining that are outside the area defining the motor nerve terminal but near perisynaptic Schwann cell nuclei, indicated by the DAPI stain (Blue). Calibration bar, 10 μm. B. All of the images shown are maximum projections of 18 images collected at 0.5 μm increments vertically through the field containing the NMJ. α-bungarotoxin (α -BTX) is shown in Red. ASIC1 antibodies are in Green. Arrows point to clusters of ASIC2a staining that are outside the area defining the motor nerve terminal but near perisynaptic Schwann cell nuclei, indicated by the DAPI stain (Blue). Calibration bar, 10 μm.

Article Snippet: For ASIC2a staining we paired the primary rabbit anti-ASIC2a antibody (ASC-012; Alomone Labs) with the American Qualex Goat Anti-rabbit FITC IgG (H±L) secondary antibody (A102FN).

Techniques: Staining

Journal: Neural Regeneration Research

Article Title: Ischemic postconditioning protects against ischemic brain injury by up-regulation of acid-sensing ion channel 2a

doi: 10.4103/1673-5374.180751

Figure Lengend Snippet: ASIC2a protein expression in the rat hippocampal CA1 region. Ischemic postconditioning strongly up-regulated ASIC2a protein expression in the rat hippocampal CA1 region after ischemic brain injury. ASIC2a: Acid-sensing ion channel 2a; GAPDH: glyceraldehye phosphate dehydrogenase.

Article Snippet: Membranes were blocked using 5% skimmed milk, incubated at room temperature for 120 minutes, washed four times (10 minutes each) in PBS-Tween, incubated with primary rabbit anti-rat ASIC2a and GAPDH polyclonal antibodies (1:5,000; Sigma, St Louis, MO, USA) at 4°C overnight, washed four times (10 minutes each) in PBS-Tween, incubated with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Sigma) at room temperature for 120 minutes, and washed four times (10 minutes each) in PBS-Tween.

Techniques: Expressing

Journal: Neural Regeneration Research

Article Title: Ischemic postconditioning protects against ischemic brain injury by up-regulation of acid-sensing ion channel 2a

doi: 10.4103/1673-5374.180751

Figure Lengend Snippet: ASIC2a mRNA expression in the rat hippocampal CA1 region in both groups

Article Snippet: Membranes were blocked using 5% skimmed milk, incubated at room temperature for 120 minutes, washed four times (10 minutes each) in PBS-Tween, incubated with primary rabbit anti-rat ASIC2a and GAPDH polyclonal antibodies (1:5,000; Sigma, St Louis, MO, USA) at 4°C overnight, washed four times (10 minutes each) in PBS-Tween, incubated with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2,000; Sigma) at room temperature for 120 minutes, and washed four times (10 minutes each) in PBS-Tween.

Techniques: Expressing